Scanning densitometry and mass spectrometry for HPTLC analysis of lipids: The last 10 years
نویسندگان
چکیده
This work is a review on HPTLC contribution to lipid analysis in complex matrices, the period from 2010 now, lapse of time where hyphenation with other techniques, especially Mass Spectrometry, has experienced an important growth. Scanning densitometry (SD)-UV/FL separated lipids, as core detection and centerpiece for hyphenation, its coupling MS using soft ionization techniques (ESI, APCI, MALDI, DESI others), are central axis this work. The occasional intercalation on-plate biological assay effect-direct (EDA) well combination SD radio-densitometry (RD) isotopically labeled-lipids also covered topics. described make possible develop strategies obtaining qualitative different levels quantitative information, including untargeted lipids species. been used for: comparative purposes; fingerprinting; semi-quantitative determination, identification species; or determination given individual lipid. Goals molecular biology biochemistry-related samples have been: preparative isolations; control purity; verification metabolic products; assays; profiles cells; products cellular metabolism; measurement enzymatic activities; monitoring transport across membranes at interfaces; dynamics. Abbreviations: AcH: acetic acid; AMD: Automated Multiple Development; APCI: Atmospheric Pressure Chemical Ionization; Cer: Ceramides; Chol: Cholesterol; CholE: Cholesteryl esters: CholS: sulfate; DCM: Dichloromethane; DESI: Desorption Electrospray DG: Diacylglycerides; E1: estrone; E2: 17-β estradiol; E3: estriol; EE2: 17-α ethynylestradiol; EDA: Effect-Direct Analysis; ESI: EtOH: ethanol; FA: Fatty Acids; FAME: acid-methyl esters; FFA: Free GalCer: Galactosyl-Ceramides; Gb3: Globotriaosylceramides; Gb4: Globotetraosylceramides; GC: Gas Chromatography; GL: Glyco-lipids; GlcCer: Glucosyl-Ceramides; GSL: Glycosphingolipids; HPTLC: High-Performance Thin-Layer IMS: Ion-Mobility Separation; LacCer: Lactosyl-Ceramides; LC: Liquid LESA: Extraction Surface LPC: Lyso-Phosphatidylcholines; LPG: Lyso-Phosphatidylglycerols; MALDI: Matrix Assisted Laser m.d.: Migration distance; MeOH: Methanol; MG: Monoacylglycerides; NL: Neutral Lipids; PA: Phosphatidic PC: Phosphatidylcholines; PE: Phosphatidylethanolamines; PG: Phosphatidylglycerols; PI: Phosphatidylinositols; PL: Glycerophospholipids; PMA: Phosphomolybdic PS: Phosphatidylserines; SD: Densitometry; SL: Sphingolipids; SM: Sphingomyelins; SQ: Semi-quantitative; Sq: squalene; SterylE: Steryl-esters; TG: Triacylglycerides
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ژورنال
عنوان ژورنال: Journal of Liquid Chromatography & Related Technologies
سال: 2021
ISSN: ['1520-572X', '1082-6076']
DOI: https://doi.org/10.1080/10826076.2020.1866600